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Web30 minute hold at -35°C. A hold to allow the whole sample to freeze after ice nucleation. -35°C to -60°C at 0.75°C/min. Final cooling step to transfer temperature, held at -60°C for 15 min before transfer. After a minimum of 24 h storage, samples were thawed rapidly in a VIA Thaw CB1000 unit (Cytiva) and recultured. crv 2015 touring WebJan 27, 2024 · The fifth through seventh numbers equate to the number of days left in the year at the time the fluid was blended. So, as the info on our bottle is "207," that means the batch of BlueDEF was made ... crv 2017 webmotors sp