Atamt1.1
WebMay 18, 2024 · Expression of AtAMT1;1 in this yeast mutant can restore its growth under low ammonium conditions, further indicating that AtAMT1;1 is capable of ammonium uptake . This 31019b yeast mutant hereafter is widely used for related yeast functional complementation due to its non-residual AMT homologous genes. WebAug 15, 2024 · AtAMT1;1, AtAMT1;3 and AtAMT1;5 are mainly expressed in the outer cell layers of the roots and root hairs, while AtAMT1;2 is mainly expressed in the endodermis cells (Kaiser et al., 2002; Loqué et al., 2006; Mayer & …
Atamt1.1
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WebJul 10, 2006 · When expressed in oocytes, AtAMT1;1 generated significant NH 4 + and CH 3 NH 3 + currents at 100 μM substrate, while K + was not transported by AtAMT1;1 (Fig. 1 A). In water-injected oocytes, no significant currents were detected, as described previously [12].External pH had little effect on NH 4 + transport by AtAMT1;1 in oocytes (Fig. 1 B), … Webthe soil to the vasculature 1,2. AtAMT1;1 and AtAMT1;3 are located in the plasma membrane of the epidermal and cortical cell layer of the root 3,4. AtAMT1;2 localization is restricted to the cortical and endodermal root cells 1. The localization and high affinity of AtAMT1;1 (In oocytes: K m = 2.7 µM 3; in plants: K m = 50.0 µM
WebDec 10, 2008 · Total RNA (20–30 μ g per lane) was separated by formaldehyde agarose gel electrophoresis, and RNA was transferred to a nylon membrane. DNA fragments carrying the ORFs of AtAMT1;1 or AtAMT1;4 were labeled with 32 P as probes for RNA hybridization. Preparation of membrane fractions and protein gel blot analysis. Total … WebJan 1, 2006 · AtAMT1;1 and AtAMT1;3 are located in the plasma membrane of the epidermal and cortical cell layer of the root (Mayer and Ludewig 2006; Loqué et al. 2006). AtAMT1;2 localization is restricted to ...
Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 WebApr 1, 2001 · Unlike AtNrt2.1, AtAmt1.1 expression is not stimulated in a nitrate reductase-deficient mutant after transfer to NO3- as sole N source, indicating that NO3- per se acts as a signal repressing transcription of AtAmt1.1. These results reveal two fundamentally different types of mechanism involved in the feedback regulation of root N acquisition ...
WebAn AtAmt1.1-Gal4 transgene using three 5x upstream activating sequence-driven reporters (luciferase, green fluorescent protein, and beta-glucuronidase) facilitated in vivo profiling …
WebJun 28, 2008 · Abstract: AtAMT1;1 was the founding member of the family of AMT/Rh ammonium transporters and accounts for about one third of the total ammonium absorption in the roots of the model plant Arabidopsis. Recent evidence suggested that at least some AMT/Rh proteins are NH 3 gas channels. In order to evaluate the transported form of … panier anti stress chien xxlWebWe have compared the biochemical properties of two different Arabidopsis ammonium transporters, AtAMT1;1 and AtAMT1;2, expressed in yeast, with the biophysical … panier anti-inflation intermarchéWebConstruction of an AtAmt1.1-Gal4 Driver System for LUC, Red-Shifted GFP, and GUS. We constructed a reporter system for nitrogen stress that fulfilled multiple criteria. LUC was used for temporal studies because it has a short half-life (Thompson et al., 1991; Millar et al., 1992; Leeuwen et al., 2000) and can be imaged noninvasively in vivo.For detailed … panier anti inflation super u listeWebJan 20, 2014 · AtAMT2-1 has ammonium transport activity similar to that of AtAMT1-1 at pH 7.5, but transport capacity of AtAMT2-1 is an order of magnitude lower than that of AtAMT1-1 at pH 6.5 . The monocot group A-I appears to have undergone an early duplication event preceding speciation in the monocots, leading to two sister clades (Figure 4A). set up appointment with quest diagnosticsWebApr 14, 2024 · Soybean (Glycine max) is a major protein and oil crop. Soybean basic region/leucine zipper (bZIP) transcription factors are involved in many regulatory pathways, including yield, stress responses, environmental signaling, and carbon-nitrogen balance. Here, we discuss the members of the soybean bZIP family and their classification: 161 … panier anti gaspi carrefour belgiqueWebThe NH 4 1 concentration needed to saturate AtAMT1;2 was much higher than for AtAMT1;1, which has a more than 10-fold higher affinity (Mayer and Ludewig, 2006; … set up a second email addressWebSep 1, 2024 · While AtAMT1–1 and 1–3 exhibited more similarity than AtAMT1–4, AtAMT1–4 was separated from all AMT1s in group B. In view of exon numbers, it was seen that the S1AMT1–2 gene contains only two exons, and this difference was observed in the phylogenetic tree, proposing that gene variations may cause this differentiation. set up audit trail in salesforce