Bioassay of cytokinin

Author: njqi

August undefined, 2024

Weband the soybean callus bioassay. The highest con-centration of cytokinin was DHZR at 35.6 ug 1-1 followed by iP 15.9 jig 1-1. The cytokinin with the lowest concentration was tZ at 0.7 pg 1-1. The total cytokinin concentration in Seasol was 0.115 mg 1-1 (Tay et al., 1985). Zeatin-O-glucoside (ZOG) and WebThe activity, uptake and metabolism of cytokinin metabolites was determined in soybean (Glycine max (L.) Merr.) callus tissue. The following activity sequence was established: zeatin riboside (ZR)>zeatin (Z)>O-glucosides of Z, ZR and their dihydro derivatives>lupinic acid (an alanine conjugate of Z)>7- and 9-glucosides of Z which were almost inactive.

(PDF) A New Bioassay for Auxins and Cytokinins

WebOct 2, 2016 · Prof. Bhagwan Deen. Physiological role: The cytokinin checks the degradation of chlorophyll and increases the RNA, DNA and protein content. It plays following role in plants: Cell division: Cytokinin promotes cell division in plant tissues in the presence of auxins. It promotes cell division in apical meristematic and cambium tissues … WebMar 1, 1974 · Cucumber cotyledon expanSIon in dark can therefore serve as a bioassay system for cytokinins. A comparison of the activity of different concentrations of kinetin and benzylaminopurine on cotyledon expansion in dark is shown in table 6 and figs. 3 to 5. It will be seen that benzylaminopurine is more active than kinetin in cucumber cotyledons ... how to start eating vegan for beginners

Wendy A. Stirk: H-index & Awards - Academic Profile Research.com

WebJun 21, 2024 · 3. CYTOKININ • Cytokinins are a class of purine-type phytohormones that promote – cell division, – shoot and root morphogenesis, – chloroplast maturation, – cell … Webshortcoming of these is that the cytokinin nucleotides, which are considered to be of major significance in cytokinin biosynthesis and metabolism [7] were not included. This paper reports on the response of 28 authentic cytokinin derivatives, including nucleotides, in the soybean callus bioassay, which is widely used in cytokinin research. 2. WebNaturally occurring cytokinins are adenine-based plant hormones. Although, the effect of various substituents at positions N1, C2, N3, N 6, N7, or N9 on the biological activity of cytokinins has been studied, the C8-substituted compounds have received little attention.Here, we report the synthesis and in vitro biological testing of thirty-one … react erp github

Cotyledon Expansion as a Bioassay for Cytokinins - JSTOR

WebHence, chlorophyll preservation test is a bioassay for cytokinin. Cytokinin promotes cell enlargement in the leaf tissues of certain plants. So, radish and bean leaf test have been … Web1-1. Introduction. We performed quantitative analysis for cytokinin using cotyledons (seed sprouts) of cucumber (Cucumis sativus L. cv.). We proposed to make E. coli produce cytokinin and needed to establish experimental system for quantitative analysis for cytokinin. Cucumber cotyledons are frequently used as samples for a simple and rapid … react env file not workingWebThe induction of shoot buds from the filamentous protonema of moss is a classic bioassay for cytokinin. While a large literature documents this response in many species of moss … how to start eating vegan

"WebMay 15, 2013 · In a bioassay the activity of a sample is tested on a sensitive cell line and the results of this activity are compared to a standard cytokine preparation. This assay is … " - Bioassay of cytokinin

Bioassay of cytokinin

Cytokinins : Physiological role and uses - agrihortieducation.com

WebThe synthetic cytokinin N6-cyclohexylmethyladenine exhibited inhibitory activity equivalent to that of 1-phenyl-3-(2-thiazolyl)-2-thiourea (PTTU), a compound known to be a potent inhibitor of dopamine-beta-hydroxylase activity. ... PTTU itself was found to exhibit cytokinin activity in the tobacco callus bioassay and to inhibit the activity of ... WebXanthium expresses a large and rapid cytokinin response which can be obtained in very small solution volumes, and which is sensitive to cytokinin concen-trations as low as 10 …

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WebJan 1, 1989 · The response of the Biosynthesis of cytokinins by A. chroococcum 969 sample observed in the bioassay was compared to the response observed by a known concentration of r-zeatin. The amount of cytokinin-like compounds present was expressed in terms of the concentration of zeatin equivalents (ZE). WebHer primary areas of investigation include Botany, Cytokinin, Food science, Horticulture and Shoot. Her study in Botany is interdisciplinary in nature, drawing from both Micropropagation and Tissue culture. Her work in the fields of Zeatin overlaps with other areas such as Seedling, Germination, Bioassay and Callus.

WebFeb 1, 1998 · The purpose of these protocols is to provide practical stepwise methods for the bioassay of cytokines using cytokine responsive cell lines. They include tables of the … WebCytokinin travels from the roots into the shoots thereby signaling bud growth in length. Cytokinin has shown the slow aging of plant organs by preventing breakdown of …

WebModifying the cytokinin content in plants is a means of improving plant productivity. Here, we report the development and biological activity of compound TD-K (1-(furan-2-ylmethyl)-3-(1,2,3-thiadiazol-5-yl)urea)which is related to thidiazuron. TD-K—which exhibited extremely high antisenescence activity in the wheat leaf bioassay—and INCYDE (2-chloro-6-(3 …

Webyet rapid, bioassay for cytokinins (7). The sensitivity and specificity of several cytokinin bioassays, including those based on leaf senescence and callus formation, have been …

WebJul 23, 2024 · Cytokinin bioassay further demonstrated that S-4893 antagonized cytokinin-induced stimulation of callus formation and inhibition of root elongation. S … react enzyme useeffectWebThe induction of shoot buds from the filamentous protonema of moss is a classic bioassay for cytokinin. While a large literature documents this response in many species of moss and for a wide range of natural and synthetic cytokinins, to date only substituted adenine cytokinins have been examined in detail. This paper shows that at least some ... how to start eating vegan dietWebThe bioassay is based on the expression in transformed tobacco ( Nicotiana tabacum) mesophyll protoplasts of a chimeric gene, consisting of the upstream sequences of the … react erp systemWebHence, chlorophyll preservation test is a bioassay for cytokinin. Cytokinin promotes cell enlargement in the leaf tissues of certain plants. So, radish and bean leaf test have been used as bioassay. Promotion of lettuce seed germination forms the basis for another bioassay for cytokinins. Dwarf maize test is a bioassay for gibberellins as they ... react error 130WebThe cucumber cotyledon greening bioassay is frequently used for detecting cytokinins. Beneficial modifications of the original technique included using 5-day-old … how to start eating rightWebCytokinin is synthesized in the roots from where they are transported to shoots by xylem tissues. Biosynthesis of purine type of cytokinin is more clearly known than of other types. Various components of purine are derived from several small molecules including formyltetrahydro folate, aspartate, CO 2, ... react error #300WebThe effect of 28 natural and synthetic cytokinins, including cytokinin nucleotides, the growth of soybean cotyledonary callus was investigated. Generally the nucleosides and … how to start eating vegetables