Web1.2 Add the corresponding azide or alkyne-containing detection reagent (see Table 3), prepared in step 1.1, directly to the Click-iT® reaction buffer (Component A) for a final concentration of 40 μM. A total volume of 100 µL of this azide- or alkyne-containing reaction buffer is required for each click detection reaction. WebAug 19, 2024 · Conjugate (β-CD (N 3) 7) with Gly-Gly-D-Pra using CuAAC in a solution of sodium ascorbate and copper (II) sulfate. The peptide will now have an alkyne group …
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WebI keep struggling with TCEP concentrations and protein (2 cysteines) conjugation with PEG-Maleimide. I performed the reaction at 10, 20 and 200µM but even at 10µM TCEP my … WebReaction Type. Reagent Type. Available for Sale. United States Globally. biotin-azide. Applied Filters: Keyword:'biotin-azide' Showing 1-10 of 10 results for "biotin-azide" within Products. Products Genes Papers Technical Documents Site Content Chromatograms. Filter & Sort. All Photos (2) Azo biotin-azide. Empirical Formula (Hill Notation): C ... tsundzukani tintswalo alice-chris mhlanga
Click-iT® Metabolic Labeling Reagents for Proteins
WebFor labeling click reaction using higher concentration of Biotin Azide increase volume of Biotin Azide solution added at Step 2.1 leaving volumes of all other reagents unchanged. 2.1 For each protein lysate sample, add the following to a 1.5 mL microfuge tube, then vortex briefly to mix. • 10 µL Biotin Azide DMSO solution. Web“click” reaction between an azide and an alkyne. In the click reaction, the modified protein is detected with a corresponding azide- or alkyne-containing dye or hapten (Figure 3, Table 3). Proteins labeled with a Click-iT® metabolic labeling reagent can be detected using the Click-iT® Cell Reaction Buffer Kit (Cat. no. C10269) for ... WebUptake of the ligand, copper, and a biotin-tagged azide in the cells was determined to be 69 2, 163 3 and 1.3 0.1 mM, respectively. After 10 minutes of reaction, the ... cells without using a chelating azide. The reaction was per-formed on cellular proteins incorporating homo-propargylglycine (HPG). This model system allowed us to phmsa reauthorization