Web【结果】FabHLH148基因CDS区全长687 bp，编码228个氨基酸，预测蛋白分子量24.89 kDa，理论等电点 (pI) 为11.65；该基因属于bHLH超家族，因其与二倍体草莓FvbHLH148序列相似度最高，所以将其命名为FabHLH148；亚细胞定位显示，FabHLH148主要定位在细胞核。 Web全身性过表达人源α-Syn-NLS转基因鼠的构建方法专利检索，全身性过表达人源α-Syn-NLS转基因鼠的构建方法属于农业林业畜牧业狩猎诱捕捕鱼专利检索，找专利汇即可免费查询专利，农业林业畜牧业狩猎诱捕捕鱼专利汇是一家知识产权数据服务商，提供专利分析，专利查询，专利检索等数据服务功能。 anderson aldridge facebook WebMar 26, 2024 · 载体质粒如图5所示。反应体系为：linearized entry clone 50ng；purified destination vector 75ng；lr clonase ii enzyme mix 0.5μl；加te(ph8.0)补足2.5μl。反应条件：25℃1h；加入0.25μl的proteinase k solution，混合混匀，之后37℃水浴10min。 WebJul 12, 2006 · Note that the stock is not a purified clone, but a mix resulting from a Gateway BP clonase reaction. Growth Requirement: Marker: gentamycin. Background: ABRC Comment: This material contains Gateway(TM) Technology owned by ThermoFisher Scientific (formerly known as Life Technologies/ Invitrogen Corporation). Distributed … bach rock guitar WebLR Clonase II Plus enzyme mix is provided in an optimized single mix of enzyme and reaction buffer, ensuring enzyme stability and easy of use with few pippetting steps for … http://thedb.cn/r/yuanyi/106.html ba christmas holidays 2022 WebDec 5, 2024 · The sequences were confirmed by sequencing and cloned into the pDONR221 (Invitrogen) vector using the Gateway BP Clonase II Enzyme Mix (Invitrogen, Carlsbad, CA, USA), resulting in pENTR vectors. The overexpression vectors were obtained using multiple recombination among the pENTR ...

WebDec 6, 2006 · The LR Clonase mix (Invitrogen) is responsible for recombination of attL sites with attR sites. Any DNA fragment of interest can be PCR amplified with primers containing attB sites and cloned into a donor vector carrying … WebThermo Fisher bp clonase mix Bp Clonase Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS … ba christmas market 2022 http://csrri.iit.edu/~howard/biol446/Lab1.bp_clonaseii_man.pdf WebJun 8, 2024 · gateway cloning 1. The Gateway cloning System, invented and commercialized by Invitrogen life technologies since the late 1990s. It is a molecular biology method that enables researchers to efficiently … ba christophe WebDec 23, 2011 · The BP CLONASE Enzyme Mix mediates the BP Reaction and contains " recombination protein Int and the E. coli-encoded protein IHF. The wild type " attB and attP recombination sites have been … WebLR Clonase II Plus Enzyme Mix. Single mix of enzyme and reaction buffer. Highest cloning efficiency for single and multiple fragment cloning. Optimized for difficult cloning … bach rock and roll http://csrri.iit.edu/~howard/biol446/Lab1.bp_clonaseii_man.pdf#:~:text=Gateway%C2%AE%20BP%20Clonase%E2%84%A2%20II%20enzyme%20mix%20is%20a,6%20months.%20For%20long-term%20storage%2C%20store%20at%20-80%C2%BAC.

Websites using the BP clonase reaction mix (Invitrogen). This BP recombination reaction results in the target sequence being flanked by attL sequences, which allows subsequent recombination with a destination vector. These options, as well as detailed protocols, are described in the Gateway cloning manual(s) available from Invitrogen’s website anderson aldridge grandfather WebGateway™ BP Clonase™ II enzyme mix includes proteinase K solution (2 µg/µl) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when … anderson alexander law firm