Ph-ncas9-ppe-v2
WebbpOsU3 pTaU3 pH-nCas9-PPE-V2/pH-ePPE Forward primer (5'-3'): + Spacer sequence + Reverse primer (5'-3'): + PBS and RT template sequence + Submit >> clear. Help … WebbThe pOsU3, pTaU3, and pTaU6 vectors are recommended for particle bombardment and protoplast transformation experiments. The pH-nCas9-PPE-V2 vector is recommended …
Ph-ncas9-ppe-v2
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WebbPlasmid pH-nCas9-PPE-V2 from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 Mar 25. pii: 10.1038/s41587-021-00868-w. … WebbPlasmid pH-A3A-PBE from Dr. Caixia Gao's lab contains the insert APOBEC3A-nCas9-NLS-UGI-NLS and is published in Nat Biotechnol. 2024 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261. This plasmid is available through Addgene.
Webb15 apr. 2024 · To construct the pH-nCas9 (H840A) vector for the control group, the M-MLV sequence of pH-nCas9-PPE was removed using a ClonExpress II Cloning Kit. The PE3 … WebbpH-nCas9-PPE-V2 Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated …
http://www.plantgenomeediting.net/ Webb24 mars 2024 · PPE-NC-v1 showed the highest editing efficiencies, ranging from 0.2% to 19.5% in both rice and wheat protoplasts, followed by PPE-NC-v2 and PPE-∆RNase H (Fig. 1f,g ). These three new PPEs...
Webb21 feb. 2024 · Cas9 protein (1 μg) and sgRNA (1 μg) were mixed with the purified target DNA (100–150 ng) in Cas9 reaction buffer (20 mM HEPES, pH 7.5, 150 mM KCl, 10 mM MgCl 2, 0.5 mM DTT) in a total volume of 20 μl, followed by digestion at 37 °C for 0.5–1 h.
http://enpcce.genetics.cas.cn/PN/CXG/ACXG/ truststoreprovider is disabledWebb24 mars 2024 · We used the ePPE to generate rice plants tolerant to sulfonylurea and imidazolinone herbicides, observing an editing frequency of 11.3% compared with 2.1% … trusts to avoid inheritance tax ukWebbPlasmid nCas9-PPE from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 May;38(5):582-585. doi: 10.1038/s41587-020 … philips b5x34aWebbLocation : Home > Faculty > Caixia Gao > Selected Publications. Selected Publications. Publications (* Corresponding author; 1 co-first author) 1. Wang S1, Zong Y1, Lin Q1, … trust storage boise idahoWebbLocation : Home > Faculty > Caixia Gao > Selected Publications. Selected Publications. Publications (* Corresponding author; 1 co-first author) 1. Wang S1, Zong Y1, Lin Q1, Zhang H1, Chai Z, Zhang D, Chen K, Qiu JL, Gao C * (2024) Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC-Cas9. philips b5x14aWebbModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites … truststrategy spring boot dependencyWebb22 juni 2024 · Answer. The dCas9, also known as endonuclease deficient Cas9, is a mutant form of Cas9, whose endonuclease activity is removed by mutating the endonuclease … philips b5x68a