Phm1 vector
Webb26 dec. 2024 · Therefore, we constructed a helper λ‐Red recombination system into the pHM1 vector, yielding pHM1‐λRed (Figure 3b), and transformed this into Xoo to improve … Webb5 juli 2024 · 110 introduction of the xopQ wildtype gene into the xopQ-strain through the pHM1 vector. 111 Notably, the xopQ D116A and xopQ Y279A mutants failed to …
Phm1 vector
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Webb19 juli 2016 · Empty pHM1 vector was also introduced into mutant strains. Spectinomycin-resistant putative complementation clones were confirmed by PCR and sequencing and … http://www.vectorhub.cn/plasmid-profile-search.html
Webbwas similarly constructed [29]. Every recombinant vector was linearized with HindIII and cloned into the pHM1 vector for genetic complementation. The hpa1pthXo1 and … Webb载体质粒Vectors and Plasmids-BioVector NTCC保藏中心-NCV2. ... Cosmid vector pHM1, complete sequence. 12,702 bp circular other-genetic . EF059993.1 GI:119034463. 3900. …
Webb12 juli 2024 · Taq polymerase ReadyMix (KAPA Biosystems, Wilmington, MA, USA) was used for all screening purposes. For cloning in the pHM1 vector (Innes et al., 1988), primers as listed in Table S2 were used for amplification of the xopX gene and its 14-3-3 protein-binding motif mutants using Phusion polymerase (Thermo Fisher Scientific). Webb1 dec. 2014 · The vector pHM1 was digested to completion with BamHI (NEB, USA) and treated with calf intestinal alkaline phosphatase (NEB, USA). The genomic and vector …
WebbBasic Vector Information; Vector Name: pHM1: Antibiotic Resistance: Spectinomycin/Streptomycin Length: 12702 bp: Type: Cosmid vector Source: Gu K, Yang …
pop in the mouthWebb24 mars 2024 · PCR产物连接EZ-T载体测序,酶切后连接pHM1,分别得到互补质粒pHM1-vgrS和pHM1-vgrR。 互补质粒分别电击转化 vgrS 和 vgrR 突变体感受态细胞,在含150 … popinthepostWebbXopK-com-R and cloned into the pHM1 vector (Qian etal., 2008) under the control of a placZ promoter. The resulting con-struct was transformed into the DxopK mutant to … pop in the noseWebbLeaves of 4-week-old N. benthamiana were infiltrated with wild-type Pss harbouring the plasmid containing the DSF synthase (pRpfF) or the empty vector (pHM1) alone or co-inoculated with Xcc EPS (xanthan; 0.5mg ml –1). The bacterial population was measured at 0, 24, and 48h post-inoculation from six 1cm 2 leaf disc areas around the ... shares in a limited company explainedWebb20 sep. 2024 · It is necessary to develop a safe and efficient siRNA delivery vector with high anti-bacterial capability. Here, three multifunctional polymeric hybrid micelles … shares in a horseWebbExcited to share the Scale and Accenture partnership! Together, we're going to be bringing AI to Enterprises and Government. pop in the shop casper wyWebb17 jan. 2024 · To modify the Agrobacterium CBE, the destination vector was replaced with the Xanthomonas-compatible vector pHM1, a broad host range plasmid derived from … shares in amazon