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Detection of pathogenic Leptospira spp. through TaqMan …?
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Detection of pathogenic Leptospira spp. through TaqMan …?
WebMORE Objective： To establish quantitative real-time PCR method for the detection of leptospira spp by using new TaqMan probe.Methods： According to the 16s rDNA to design a pair of primers and probes,and then take the nuclear acid of standard strain of leptospira spp as template to set up quantitative real-time PCR on detector（ABI 7500 ... WebAug 1, 2013 · Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene. ... Detection of pathogenic leptospires by real-time quantitative PCR. J Med Microbiol, 54 (2005), pp. 45-49. ... A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp. BMC Infect Dis, 2 (2002), p. 13. astro boy 1980 english WebMar 20, 2024 · A novel multiplex qPCR assay to enable the specific and selective detection of the whole group of infectious Leptospira spp. Leptospirosis is recognized as the most globally widespread reemerging zoonosis and represents a serious threat for both human and animal health. Indeed, leptospirosis is linked to more than 60,000 human deaths … WebAug 11, 2010 · The recovery of Leptospira DNA was assessed in triplicates by the quantitative PCR (qPCR) assay, described below. A total of 30 qPCR assays was made for each evaluated method. All DNA extractions were performed simultaneously. ... Smythe LD, Smith IL, Smith GA, Dohnt MF, Symonds ML, et al. (2002) A quantitative PCR … astro boy 1980 english cast WebQuantitative PCR design and validation A homologous region on the 16S rRNA gene of Leptospira was targeted for quantitative PCR (qPCR) assay design to ensure broad species detection. Vector NTI soft-ware (Thermo Fisher Scientific) was used to confirm the alignment of multiple Leptospira sequences. The WebThis study assessed the relative performance of different quantitative assays (SYBR green and TaqMan chemistries) using different primer sets for the detection of DNA from … astro boy 1980 WebJan 1, 2012 · This chapter presents a qPCR assay that targets lipL32, a gene which is present only in pathogenic Leptospira spp. This assay utilizes a TaqMan probe and instructions for use on either the Lightcycler 1.2 (Roche Diagnostics, Indianapolis, IN) or the ABI 7500 (Applied Biosystems, Foster City, CA) are provided. Key words. Leptospira; …
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WebJul 7, 2002 · In this study, we developed a real-time polymerase chain reaction (PCR) assay using a TaqMan probe targeting lipL32, which is present only in pathogenic Leptospira … WebApr 4, 2002 · The PCR assay can be applied to either blood or urine samples and does not rely on the isolation and culture of the organism. Capability exists for automation and high throughput testing in a clinical laboratory. It is specific for Leptospira and may discriminate pathogenic and non-pathogenic species. The limit of detection is as low as two cells. 802.1 x cisco switch WebAug 1, 2002 · The PCR assay can be applied to either blood or urine samples and does not rely on the isolation and culture of the organism. Capability exists for automation and high throughput testing in a... 802.1 x cisco active directory WebJul 1, 2009 · In this study, we developed a real-time polymerase chain reaction (PCR) assay using a TaqMan probe targeting lipL32, which is present only in pathogenic Leptospira spp. Using Leptospira interrogans serovar Icterohaemorrhagiae DNA, the lower limit of detection was found to be 20 genomic equivalents/reaction with a 95% cutoff value. WebLeptospirosis is caused by pathogenic Leptospira spp., which can be found in nature among domestic and wild animals. In Colombia, the Macaregua cave is known for its bat richness; thus, because bats are reservoir hosts of human microbiological pathogens, we determined if the Macaregua cave bats harbored Leptospira in the wild. A total of 85 … 802.1 x computer authentication failed windows 10 WebMar 22, 2024 · Leptospirosis is a re-emergent zoonotic bacterial disease associated with renal and hepatic injury. In free-roaming cats in some regions, a high prevalence of Leptospira antibodies has been identified, and pathogenic leptospires have been detected in renal tissue, indicating that they may play a role in Leptospira epidemiology. The …
WebJun 1, 2024 · Cells presenting immunogenic peptides derived from pathogen- and tumor-associated antigens can be specifically targeted by active or passive immunization strategies. ... the TaqMan ® procedure describes one such fluorescent methodology for performing Quantitative PCR. ... The precise structure of regulatory sequences can vary … WebApr 6, 2011 · In this study, four quantitative real-time PCR (qPCR) assays (SYBR green and TaqMan chemistries) targeting the secY, lfb1, and lipL32 genes were evaluated as diagnostic assays. In our hands, these assays can detect between 10 (2) and 10 (3) bacteria/ml of pure culture, whole-blood, plasma, and serum samples. astro boy 1980 intro WebThis chapter presents a qPCR assay that targets lipL32, a gene which is present only in pathogenic Leptospira spp. This assay utilizes a TaqMan probe and instructions for … WebDec 2, 2024 · The lipl32 TaqMan real-time PCR assay was able to detect DNA from all the pathogenic Leptospira spp. tested, while none of the intermediate and saprophytic … astro boy 1980 english sub WebJul 1, 2009 · In this study, we developed a real-time polymerase chain reaction (PCR) assay using a TaqMan probe targeting lipL32, which is present only in pathogenic Leptospira … WebResults and Conclusions: The PCR assay can be applied to either blood or urine samples and does not rely on the isolation and culture of the organism. Capability exists for … 802.1 x certificate based authentication Web9278852482056946_manuscript - Free download as PDF File (.pdf), Text File (.txt) or read online for free. useful info
WebApr 4, 2002 · The PCR assay can be applied to either blood or urine samples and does not rely on the isolation and culture of the organism. Capability exists for automation and … astro boy 1980 english dub WebJul 8, 2002 · The polymerase chain reaction (PCR) has been used to detect a large number of microorganisms, including those of clinical significance. The sensitivity of PCR often … astro boy 1980 season 1 episode 1