WebMar 3, 2024 · 2.1. Construction of pBiFC and pBabe Vectors. The coding sequences for human Bcl-2, Bcl-X L, Mcl-1, Bim, Puma, Noxa, Bax, Bak, and TOM20 were subcloned by standard PCR strategies [] into pBiFC plasmids modified to contain the sequences coding for VN (amino acids 1–173) or VC (amino acids 155–238) fragments of Venus protein and …
Analyzing protein-protein interactions from affinity purification …
WebAbstract. We present 'significance analysis of interactome' (SAINT), a computational tool that assigns confidence scores to protein-protein interaction data generated using affinity … WebSignificance Analysis of INTeractome (SAINT) is a software package for scoring protein-protein interactions based on label-free quantitative proteomics data (e.g., spectral count … inail thiene
[PDF] Analyzing Protein‐Protein Interactions from Affinity …
Label-free quantification in this work was based on spectral counting. Spectral counts are the sum of every successful instance of sequencing a peptide from a particular protein by mass spectrometry, including redundant spectra. With proper normalization, spectral counts can be used as a quantitative … See more This section describes the generalized statistical modeling framework for the datasets with and without control purifications (Fig. 1a,b). In both cases, the spectral … See more CompPASS7,10 calculates two different scores. First, Z score is constructed by mean centering and scale normalization in the conventional Z statistic, where … See more The source C code and a user manual for the generalized SAINT model described in this work (SAINT 2.0) can be downloaded from http://saint … See more WebSAINT的介绍. SAINT（Significance Analysis of INTeractome）是一种概率方法，用于在亲和纯化-质谱 (AP-MS)实验中对阴性对照的诱饵-猎物相互作用进行打分，从而获取与靶向 … WebMar 3, 2024 · Note that for analysis with significance analysis of interactome (SAINT), only proteins with iProphet protein probability R ≥ 0.95 and two unique peptides are considered. SAINT Analysis and Data Visualization. SAINTexpress (version 3.6.3) 25 was used to score proximity interactions from DDA data. inail tiburtina