WebBoth amplicons (the 53BP1 and mCherry) were later assembled with the pre-restriction cut (SpeI and KpnI) pBluescript II SK(+) using NEBuilder HiFi DNA Assembly Cloning Kit … WebTransformation: NEB 5-alpha Competent E. coli (High Efficiency, NEB #C2987) provided with the NEBuilder HiFi DNA Assembly Cloning Kit are recommended for use for assembled products of less than 15kb. It is also possible to use other NEB competent E. coli strains, with the exception of BL21, BL21 (DE3), Lemo21 (DE3), Nico21 (DE3), and … box trailer hire WebMar 10, 2024 · Blasticidin S deaminase (BSD) selection marker and a destabilization domain (DD) were cloned into lentiCRISPRv2 (Addgene, 52961) using NEBuilder HiFi DNA Assembly in a stepwise manner. Cloning of BSD was performed by assembling three components: two PCR-amplified pieces of lentiCRISPRv2 and one PCR-amplified piece … WebFeb 17, 2015 · For use with NEBuilder ® Hifi DNA Assembly Cloning Kit ( NEB #E5520 ), NEBuilder HiFi DNA Assembly Bundle for Large Fragments ( NEB #E2623 ) NEBuilder® HiFi DNA Assembly Chemical Transformation Protocol (E2621, E5520, E2623) NEB box trailer dump bed WebFeb 17, 2015 · NEBuilder ® HiFi DNA Assembly Chemical Transformation Protocol (E2621, E5520, E2623). For use with NEBuilder ® Hifi DNA Assembly Cloning Kit (), NEBuilder HiFi DNA Assembly Bundle for Large Fragments (), and NEBuilder HiFi DNA Assembly Master Mix Protocols.io also provides an interactive version of this protocol … WebLimited Warranty: The NEBuilder ® HiFi DNA Assembly Master Mix and NEBuilder HiFi DNA Assembly Cloning Kit are warranted to perform according to specifications stated on the certificate of analysis. No other warranty is made, whether express or implied, including any warranty of merchant ability or fitness for a particular purpose. This war- box trailer for sale scotland Web1 day ago · A site-directed mutagenesis kit was applied to precisely induce point mutations (Transgene, Beijing, China). An NEBuilder HiFi DNA Assembly kit was used for fragment assembly. The cloned, mutated and assembled sequences of the components or full-length BEs were confirmed by Sanger sequencing before further vector construction (Sangon …

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